A Comparative Study on Phytochemical Screening and Antioxidant Activity of Aqueous Extract from Various Parts of Moringa oleifera

  • Awalul Fatiqin Department of Biology, Islamic State University Raden Fatah, Palembang 30126, South Sumatra, Indonesia http://orcid.org/0000-0001-7799-2835
  • Hanif Amrulloh Department of Islamic Primary School Teacher Education, Institute for Islamic Studies Ma’arif NU (IAIMNU) Metro Lampung, Metro 34111, Lampung, Indonesia http://orcid.org/0000-0001-7458-9258
  • Ike Apriani Department of Biology, Islamic State University Raden Fatah, Palembang 30126, South Sumatra, Indonesia
  • Aneke Lestari Department of Biology, Islamic State University Raden Fatah, Palembang 30126, South Sumatra, Indonesia
  • Berta Erawanti Department of Biology, Islamic State University Raden Fatah, Palembang 30126, South Sumatra, Indonesia
  • Ade Saputri Department of Biology, Islamic State University Raden Fatah, Palembang 30126, South Sumatra, Indonesia
  • Maghfiro Gita Department of Biology, Islamic State University Raden Fatah, Palembang 30126, South Sumatra, Indonesia
  • Maeni Fitrianti Department of Biology, Islamic State University Raden Fatah, Palembang 30126, South Sumatra, Indonesia
  • Ramachandra Rao Sathuluri Department of Reproductive Biomedicine, The National Institute of Health and Family Welfare, New Delhi 110067, India https://orcid.org/0000-0002-4135-4054
  • Yehezkiel Steven Kurniawan Ma Chung Research Center for Photosynthetic Pigments, Universitas Ma Chung, Malang 65151, East Java, Indonesia http://orcid.org/0000-0002-4547-239X
  • Rr. Mini Sari Wulan Department of Biology Education, Islamic State University Raden Fatah, Palembang 30126, South Sumatra, Indonesia
  • Muhammad Saqib Khan Department of Environtmental Science, COMSATS University Islamabad, Abbottabad 22060, Pakistan https://orcid.org/0000-0002-0897-7436
Keywords: Moringa oleifera, phytochemical, phenolic content, antioxidant


In this work, we reported a comparative study on the phytochemical screening and antioxidant activity of aqueous extract from different parts of Moringa oleifera plant, i.e. leaves, twigs, stem barks, and woods. The phytochemical content of each extract was screened through qualitative analysis and followed by quantitative analysis for the determination of total phenolic content and total flavonoid content. The results showed that different parts of Moringa oleifera plant would give different extracted natural products. Either leaf or twig part of Moringa oleifera contained alkaloids, flavonoids, saponins, carbohydrates, polyphenols, proteins, amino acids, and phenolics. Meanwhile, only flavonoids, carbohydrates, and phenolics were found in the aqueous extract of the wood part of Moringa oleifera. In agreement to the phytochemical assay, the aqueous extract of leaf part of Moringa oleifera gave the highest total phenolic content (684 ± 16.7 µg GAE mL-1) and the highest flavonoid content (514 ± 26.1 µg CE mL-1) than other parts. Consequently, the leaf extract exhibited the highest antioxidant activity (64.6 ± 0.69%) which is close to the antioxidant activity of ascorbic acid (71.7 ± 0.56%) as the positive control. This finding is critical for the utilization of Moringa oleifera leaf part as the most potent source of antioxidant agents in the future.


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How to Cite
Fatiqin, A., Amrulloh, H., Apriani, I., Lestari, A., Erawanti, B., Saputri, A., Gita, M., Fitrianti, M., Sathuluri, R. R., Kurniawan, Y. S., Wulan, R. M., & Khan, M. S. (2021). A Comparative Study on Phytochemical Screening and Antioxidant Activity of Aqueous Extract from Various Parts of Moringa oleifera. Indonesian Journal of Natural Pigments, 3(2), 43. https://doi.org/10.33479/ijnp.2021.03.2.43
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